Antigen

Antigen Class

TFs and others

Antigen

RTF1

Cell type

Cell type Class

Digestive tract

Cell type

DLD-1

Primary Tissue

Colon

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

DLD-1

cell line

DLD-1

cell type

Colorectal adenocarcinoma

genotype

SPT6-AID OsTIR1

treatment

Vehcle 2h

chip antibody

RTF1 (Bethyl # A301-329A)

Sequenced DNA Library

library_name

GSM6102813

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq in human DLD1 cells, ~30 million cells were crosslinked with 1% paraformaldehyde in PBS for 10 min at r.t. Spike-in mouse embryonic fibroblast fixed cells were added to samples. Chromatin was sonicated with the Covaris E220. Immunoprecipitations were carried out with antibodies and Dynabeads Protein G. For ChIP-seq, DNA libraries were prepared by the HTP Library Preparation Kit for Illimina (KAPA).

Sequencing Platform

instrument_model

Illumina NovaSeq 6000

hg38

Number of total reads

39500172

Reads aligned (%)

88.0

Duplicates removed (%)

34.9

Number of peaks

758 (qval < 1E-05)

hg19

Number of total reads

39500172

Reads aligned (%)

87.7

Duplicates removed (%)

35.6

Number of peaks

911 (qval < 1E-05)